anti il17a  (Miltenyi Biotec)

Journal: PLOS ONE

Article Title: A glucan-particle based tularemia subunit vaccine induces T-cell immunity and affords partial protection in an inhalation rat infection model

doi: 10.1371/journal.pone.0294998

Figure Lengend Snippet: F344 rats were vaccinated with GP vaccine combinations that included FTT0814, LPS and imiquimod (IMQ) adjuvant. Additional group included of rats vaccinated with the live attenuated vaccine F. tularensis LVS, sham-inoculated with PBS and empty GPs. Splenocytes were isolated 6 weeks after the final vaccination and stimulated for 20 hours with each of the antigen shown on the x-axis of the bar graphs. Brefeldin A was included in the last 4 hours of culture. A minimum of 1x105 singlet, live, lymphocyte cell events were acquired for each sample. The stacked bar graphs show the mean percentage of either CD4+ or CD8+ cells expressing either only IFNγ (blue bars), only IL17 (green bars) or both IFNγ and IL17 (red bars) with error bar representing the SEM for the group. Responses are only reported for those animals/samples where post-processing functional viability was confirmed by measurement of mitogen (ConA) responsiveness.

Article Snippet: Fixed cells were permeabilised in BD Biosciences Permeabilistation Buffer and then stained intracellularly for 30 minutes at 4°C using anti-rat antibodies IFNγ-PE (clone DB-1, BD Biosciences) and IL17A-APC (clone eBio17B7, eBioscience).

Techniques: Adjuvant, Isolation, Expressing, Functional Assay

Journal: Cell reports

Article Title: An axon-T cell feedback loop enhances inflammation and axon degeneration

doi: 10.1016/j.celrep.2024.113721

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Mouse IL17A APC , eBioscience , RRID: AB_763580.

Techniques: Control, Blocking Assay, Recombinant, Cream, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software